Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® β-Catenin siRNA I #6225 (+) or SignalSilence ® β-Catenin siRNA II (+), using β-Catenin (6B3) Rabbit mAb #9582 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-catenin antibody confirms silencing of β-catenin expression, while the α-tubulin rabbit mAb is used is used as a loading control.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® β-Catenin siRNA I #6225 (+) or SignalSilence ® β-Catenin siRNA II (+), using β-Catenin Antibody #9562 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The β-catenin antibody recognizes both β-catenin and γ-catenin and demonstrates the specificity of β-catenin expression silencing. The α-tubulin rabbit mAb is used as a loading control.